Characterization of Aquatic Biofilms with Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Determination of DNA content of aquatic bacteria by flow cytometry.
The distribution of DNA among bacterioplankton and bacterial isolates was determined by flow cytometry of DAPI (4',6'-diamidino-2-phenylindole)-stained organisms. Conditions were optimized to minimize error from nonspecific staining, AT bias, DNA packing, changes in ionic strength, and differences in cell permeability. The sensitivity was sufficient to characterize the small 1- to 2-Mb-genome o...
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Environmental research on aquatic microorganisms, such as phytoplankton, aquatic bacteria, and aquatic viruses, often takes advantage of their natural chlorophyll, phycobilins, and other intrinsic fluorescent pigments. Although epifluorescence microscopy is still used frequently, flow cytometry has become a key research tool due to its ability to provide multiparametric analysis at the single-c...
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Biofilms serve essential ecosystem functions and are used in different technical applications. Studies from stream ecology and waste-water treatment have shown that biofilm functionality depends to a great extent on community structure. Here we present a fast and easy-to-use method for individual cell-based analysis of stream biofilms, based on stain-free flow cytometry and visualization of the...
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With the exception of hematological malignancies, flow cytometry (FC) is infrequently applied as an ancillary tool in the diagnosis of malignant effusions in most institutions. However, FC may be effectively used to differentiate between epithelial cells, mesothelial cells and leukocytes using antibodies against both cell surface and intracellular proteins, offering the advantage of quantitativ...
متن کاملMalignant Nonhematological Effusion Characterization by Flow Cytometry.
With the exception of hematological malignancies, flow cytometry (FC) is infrequently applied as an ancillary tool in the diagnosis of malignant effusions in most institutions. However, FC may be effectively used to differentiate between epithelial cells, mesothelial cells and leukocytes using antibodies against both cell surface and intracellular proteins, offering the advantage of quantitativ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/57655